Advice 1: Transcript of bacterial stool

Feces (stool) is the contents of the distal colon, which is released during defecation. It consists of the remnants of digested food, residues of digestive juices, micro-organisms (95% of which is dead), water. To study the bacterial composition of the feces there is a bacterial stool test (fecal dispropo, the cultures). This study aimed at the detection of the causative agents of intestinal infections.

Transcript of bacterial stool

Bacterial stool test: concept, indications for



Bacterial feces analysis aimed to determine the intestinal microflora and detection of pathogenic bacteria, viruses.

Intestinal microflora rich in various microorganisms. They are divided into obligate group of bacteria (lactic acid bacteria and bifidobacteria, E. coli, etc.), plus (staphylococci, fungi) and transient (opportunistic pathogens). Obligate the group is 95% - 98% of the total microflora. Its functions include synthesis of b vitamins and vitamin K, fat metabolism, water-salt metabolism, thermal exchange, elimination of toxins, the formation of immunity, etc. At infringement of balance of microflora manifests itself dizbakterioz. When injected into the intestine of pathogenic microorganisms (e.g. Salmonella) will develop an infectious disease. In order to determine the nature of dizbakterioz or a disease, is assigned a bacterial stool cultures (bacanales).

Indications for bacterial analysis:

- babies of mothers who suffer from bacterial vaginitis and who are in the nursing home;
- children who have prolonged indigestion, frequent colds;
- long-term antibiotic therapy, hormonal therapy, long-term treatment with NSAIDs;
after infectious diseases of the gastrointestinal tract, or parasitic infestations;
- during chemotherapy and radiotherapy in cancer patients;
- with immunodeficiency.

Decoding results bachanalia



Standards of cultures differ in younger children, and older adults. One Bactriana cell CFU (colonialera unit), these units is measured by concentration or number of microorganisms in the investigated environment. Consider SOME different ways: counting under a microscope in a certain amount of sample, separation of the solid environment on the sector and counting the number of microbes in one sector, etc. are Recorded in degrees (10*10, 10*8, etc.), referred to as CFU/ml CFU/square centimeter, etc.

Bifidobacteria represent 95% of gut bacteria, take part in sinetta b vitamins, shaping the immune system. The reason for the decline in their number could be improper diet and drug therapy, intestinal infections and chronic diseases gastrointestinal tract, fermentopathy, and immune diseases, changes in climatic zones and stress.

The vitals:

- children under one year: 10*10-10*11
- older children: 10*9-10*10
- adults: 10*8-10*10

Lactobacilli constitute 4%-6%, maintain the PH in the intestine, substances produced by them, are involved in the destruction of pathogenic bacteria, lactase. The reason for the decline in their number could be incorrect nutrition,drug therapy, intestinal infections, chronic diseases of the gastrointestinal tract, stress.

The vitals:

- children under one year: 10*6-10*7
- older children: 10*7-10*8
- adults: 10*6-10*8

The Escherichia are involved in the formation of vitamins, shaping the immune processing of sugars. They appear in the body from birth and stay there throughout a person's life. The reason for the decline in their number could be improper nutrition, antibiotic therapy, intestinal infection and helminthiasis.

- children under one year: 10*6-10*7
- older children: 10*7-10*8
- adults: 10*6-10*8

Bacteroides help to process fats. Not are sown in babies, only children from 8-9 months. Antibiotic therapy and intestinal infections can lead to a decrease in their number in the intestine.

- children under one year: 10*7-10*8
- older children: 10*7-10*8
- adults: 10*7-10*8

Propionobacteria are involved in the processing of carbohydrates and dairy proteins, as well as help to control the normal PH in the intestine. Getting into other organs and cause inflammation.

- children under one year: 10*3-10*5
- older children: 10*5-10*6
- adults: 10*5-10*6

Enterococci are involved in the processing of carbohydrates, production of vitamins, the formation of local immunity in the gut). In excess quantities cause a number of diseases. The reasons for the increase in their number could be low immunity, food Allergy, antibiotic therapy, poor diet, reduced the number of Escherichia, helminthiasis.

- children under one year: 10*5-10*7
- older children: 10*5-10*8
- adults: 10*5-10*8

Saprophytic staphylococci are non-pathogenic (hegemonically and epidermal) and pathogenic (plasmahouse, hemolytic and Staphylococcus). Golden causes a toxic infection. Pathogenic stafilokokkove in cultures in norm should not be. They get into the system from the environment at the "help" dirty hands, poor quality food, healthcare-associated infections.

- children up to years: ≤104
- older children: ≤104
- adults: ≤104


Clostridia are involved in the processing of proteins, the result is produced skatole and indole. In small quantities these compounds stimulate peristalsis in the large can cause putrefactive dyspepsia. Large amounts of protein foods may lead to pathological growth of clostridia.

- children up to years: ≤103
- older children: ≤105
- adults: ≤105


Fungi of the genus candida (Candida). If you increase them in the gut appears fermentative dyspepsia, with the increase in their number in other organs develop various candidiasis. The overgrowth of Candida can cause large amounts of carbohydrates in the diet, antibiotic therapy, diabetes, pregnancy, stress.

- Children under one year ≤103
The older children ≤104
Adults ≤104


Pathogenic enterobacteria (Salmonella, Shigella) bacterial culture test detected normal.

Rules for collecting stool for bacterial analysis



A few days before collection to cancel the antibiotics (not to start antibiotics before it is put bacanales), NSAIDs, laxatives, antidiarrheal and anthelmintic drugs. Not to put before the enema. To warn the doctor about the medicines you are taking, visited shortly before the date of analysis countries (Kale can be specific fungi or parasites).

To collect the feces you need into a sterile container. He is going to a special spatula from the three different locations of one portion of feces, approximately a third of a standard container (2 teaspoons). Cal should not be in contact with urine, water and cleaning products or detergents.

Ideally, feces should be delivered to the laboratory warm ( no more than 40 minutes after defecation), but acceptable delivery time up to 3 hours. In emergency cases you can store the container with feces in the refrigerator for up to 8 hours but it is worth remembering that for long periods of time can affect the result.

Advice 2: How to pass tests for enteric group

In the human gut lives a huge amount of bacteria, both harmless and pathogenic. If immunity is reduced, opportunistic bacteria become aggressive and cause injury. In addition, there are bacteria that are harmful initially. If they enter the intestine, can cause diarrhea and more serious problems. To find out if in the gut "pests", prescribe stool for enteric group.
The analysis of a feces on intestinal group is a very important study
You will need
  • sterile container for collecting feces
Instruction
1
If you have any bowel problems, be sure to go to the doctor, tell us about your problem and ask them to prescribe you a stool sample for enteric group.
2
Find out and record exactly where and what time you need to bring in a stool sample. Ask your doctor what foods you should not eat on the eve of the collection analysis.
3
Go to the drugstore and buy a special container to collect feces. This is a normal plastic jar with airtight screw-on lid with convenient spoon to collect feces. The container sterile, so open it unnecessarily is not necessary.
4
If you are unable to find a container to collect feces in pharmacies (it happens, but very rarely, or you have not had time for this), take any glass container with a lid. You can use a jar of mayonnaise or other product. Thoroughly wash and boil utensils. To do this, put on the bottom of the pan a cloth napkin, it is a jar, cover with water. Bring the water to a boil, wait 5 minutes. Then remove the jar, place it on a clean towel and wait until dry.
5
On the appointed day, collect fresh feces and put in a container. Enough of volume equal to a couple teaspoons. Do not perform hygienic procedures before collection, otherwise, soap and other products can get into the material. Do not store the tests for a long time – the faster you will bring them to the lab, the better. Before leaving the house put a container in the fridge.
6
Giving samples to the lab, attach to the jar the directions to avoid confusion. Ask where and when you can pick up the results.
Note
The results of the analysis of a feces on intestinal group, you will get not earlier than in a week. This is the time necessary for the bacteria are given a "harvest".
Useful advice
The stool test is prescribed not only to patients but also to healthy people as a preventive measure.
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