Preparation for assay
During pre-training, for 5-8 hours before taking the smear, typically the doctors canceled all the therapeutic procedures and the use of prescribed drugs. Emitted from the eye material taken immediately after sleep to wash out of the places of greatest accumulation in the conjunctiva.
The methodology of the
According to the method, a swab is taken separately with each eye dry with a sterile tampon, a probe or a platinum loop which is heated for sterilization in the flame of a spirit lamp red. Lower eyelid pulled and cooled loop or swab is carried out on the lower transition crease. Removing the upper eyelid of his transitional fold collect a lump of mucus.
A clean glass slide is wiped with alcohol and a thin layer applied to it taken from eye material. The dried smear is fixed above the burner and for convenience denote contours with steklograf.
Sowing is carried out by lowering the loop taken in agar or broth in a sterile test tube over the flame of the burner, which closed with a stopper.
The material for the smear can be stored at temperatures not exceeding 8° C and it is recommended to promptly deliver to the lab.
Types of analysis of eye secretions
The complete analysis of the smear from the eye includes the following types:
Cytological analysis, in which an infectious or allergic nature of the inflammation revealed by examining stained smears under the microscope.
Microscopy analysis, in which a magnifying device is studied the microbial composition of stained smears from the eye. This method quickly and accurately determines the streptococci and staphylococci, Escherichia coli and diphtheria bacilli, chlamydia, gonococci, and fungi of the genus Aspergillus.
Bacteriological analysis consists in sowing stroke on a nutrient medium to grow colonies of pathogens inflammation.
On the conjunctiva in any case inhabits a number of conditionally pathogenic microorganisms, therefore, to determine the nature of diseases it is necessary not only to allocate everything in a smear of the germs, but also to calculate their exact number.
Bacteriological method is effective when you want to confirm that purulent inflammation initiated by excessive growth of one of the members of the native microflora of the eye.
Clarification of level of sensitivity to antibacterial preparations.
Advice 2: How to take the analysis on Staphylococcus aureus
Staphylococcus belongs to the gram-positive bacteria. Currently, there are 27 varieties of them. About 14 species of Staphylococcus found on the skin and mucous membranes of humans, however, the cause of the disease can only some of them. To identify this bacterium in the organism used for microbiological research method.
Some types of staph dangerous for the body that can weaken the immune system. Bacteria act directly against cells of the immune system and facilitate access to other pathological microorganisms. Staphylococcus may cause an allergic reaction, its enzymes are negatively affecting other cells, and the toxins poisoning the body. Especially dangerous these bacteria can be for pregnant women, staph infection leads to damage of internal organs, fetus, neonates manifested pustular wounds, tumors.
Analysis of Staphylococcus aureus is administered in the following cases: if you suspect an infection, caused by Staphylococcus (tonsillitis, pharyngitis), on the bacteriocarrier, prior antibiotic treatment against infections caused by S. aureus in nosocomial infections in the period of regular preventive examinations of nurses and workers of public catering, in pregnancy. To detect staph in the body, passing the biological samples. The study was conducted with a microbiological method. For analysis use the following material: a swab from the nose, oropharynx, breast milk, a single portion of urine, sputum, swab of conjunctiva, ear discharge, urogenital swab, rectal swab, faeces.
For 8-12 hours prior to the collection of sputum consume a large amount of liquid (water). Do not take diuretics for 2 days before urine collection. Eliminate the laxatives and other medicines, the introduction of rectal ointments, suppositories, limit medications that affect intestinal motility (drugs pilocarpine, belladonna, etc.) and the color of the chair (drugs bismuth, iron, barium sulfate) for 3 days prior to collection of feces. Doing research before taking antibiotics and other antimicrobial drugs.
Women should take the urogenital swab or urine to one month or 2 days after. Breast milk collected from the left and right breast in different containers. Men are advised not to urinate for 3 hours before passing urine or urogenital smear. The output indicators analysis are reflected in the number of colony forming units in 1 ml of the material. In healthy people with strong immunity may be detected carriage of Staphylococcus aureus. In this case, the indicator of the result of the analysis can be up to 10 CFU/ml in people with lowered immunity, this figure could reach more than 10 CFU/ml, in this case Staphylococcus aureus may cause severe inflammatory process.